Benzohydroxamic acid induces polyspermic fertilization in the sea urchin Arbacia punctulata

Herbert Schuel; Regina Schuel

doi:10.1016/0309-1651(87)90025-7

Benzohydroxamic acid induces polyspermic fertilization in the sea urchin Arbacia punctulata

Herbert Schuel
, Regina Schuel

Department of Pathology and Anatomical Sciences

SUNY Buffalo
The University of Chicago

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Benzohydroxamic acid (BHA) is a competitive inhibitor of the sea urchin sperm peroxidase. We now report that addition of BHA to fertilization cultures of Arbacia punctulata promotes polyspermy. This effect is dose and sperm density dependent. The cortical reaction (elevation of the fertilization envelope) is not retarded by BHA. BHA must be added to the cultures before the eggs complete the cortical reaction at 60 sec post insemination in order to induce polyspermy. Since sea urchin eggs release H₂O₂ during the cortical reaction at fertilization, these findings support our hypothesis that the sperm peroxidase has a functional role in helping to prevent polyspermy.

Original language	English
Pages (from-to)	189-196
Number of pages	8
Journal	Cell Biology International Reports
Volume	11
Issue number	3
DOIs	https://doi.org/10.1016/0309-1651(87)90025-7
State	Published - Mar 1987

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title = "Benzohydroxamic acid induces polyspermic fertilization in the sea urchin Arbacia punctulata",

abstract = "Benzohydroxamic acid (BHA) is a competitive inhibitor of the sea urchin sperm peroxidase. We now report that addition of BHA to fertilization cultures of Arbacia punctulata promotes polyspermy. This effect is dose and sperm density dependent. The cortical reaction (elevation of the fertilization envelope) is not retarded by BHA. BHA must be added to the cultures before the eggs complete the cortical reaction at 60 sec post insemination in order to induce polyspermy. Since sea urchin eggs release H2O2 during the cortical reaction at fertilization, these findings support our hypothesis that the sperm peroxidase has a functional role in helping to prevent polyspermy.",

author = "Herbert Schuel and Regina Schuel",

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AU - Schuel, Herbert

AU - Schuel, Regina

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N2 - Benzohydroxamic acid (BHA) is a competitive inhibitor of the sea urchin sperm peroxidase. We now report that addition of BHA to fertilization cultures of Arbacia punctulata promotes polyspermy. This effect is dose and sperm density dependent. The cortical reaction (elevation of the fertilization envelope) is not retarded by BHA. BHA must be added to the cultures before the eggs complete the cortical reaction at 60 sec post insemination in order to induce polyspermy. Since sea urchin eggs release H2O2 during the cortical reaction at fertilization, these findings support our hypothesis that the sperm peroxidase has a functional role in helping to prevent polyspermy.

AB - Benzohydroxamic acid (BHA) is a competitive inhibitor of the sea urchin sperm peroxidase. We now report that addition of BHA to fertilization cultures of Arbacia punctulata promotes polyspermy. This effect is dose and sperm density dependent. The cortical reaction (elevation of the fertilization envelope) is not retarded by BHA. BHA must be added to the cultures before the eggs complete the cortical reaction at 60 sec post insemination in order to induce polyspermy. Since sea urchin eggs release H2O2 during the cortical reaction at fertilization, these findings support our hypothesis that the sperm peroxidase has a functional role in helping to prevent polyspermy.

UR - https://www.scopus.com/pages/publications/0023300046

U2 - 10.1016/0309-1651(87)90025-7

DO - 10.1016/0309-1651(87)90025-7

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SN - 0309-1651

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EP - 196

JO - Cell Biology International Reports

JF - Cell Biology International Reports

IS - 3

ER -

Benzohydroxamic acid induces polyspermic fertilization in the sea urchin Arbacia punctulata

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