Abstract
Lipopolysaccharide endotoxin and interferon-γ induced inducible nitric oxide synthase (iNOS) protein expression and nitrite/nitrate formation in microvascular endothelial cell cultures (ECs) derived from rat skeletal muscle. Pretreatment of ECs with ascorbate accumulated a large amount of ascorbate inside the cells and consequently decreased both intracellular oxidant level and iNOS induction. These effects of ascorbate were abolished in the presence of exogenous superoxide generated by xanthine oxidase/xanthine plus catalase but were not altered when N-nitro-L-arginine methyl ester was applied to inhibit nitric oxide synthesis. Ascorbate also attenuated the activation of transcription factor IRF-1 but not NFκB. These results indicate that ascorbate inhibits iNOS expression in ECs by an antioxidant mechanism independent of both NFκB activation and the reported negative feedback effect of nitric oxide.
| Original language | English |
|---|---|
| Article number | 26140 |
| Pages (from-to) | 122-126 |
| Number of pages | 5 |
| Journal | FEBS Letters |
| Volume | 520 |
| Issue number | 1-3 |
| DOIs | |
| State | Published - 2002 |
Keywords
- Ascorbate
- Endothelial cells
- Inducible nitric oxide synthase
- Oxidant
- Sepsis
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