Abstract
This article focuses on the imaging tools which cell biologists and pathologists use to collect and connect structural and molecular data from biological systems. These tools are varied and each imaging method offers a very different representation of the data which resides within the biological systems under study. An understanding of cellular imaging methods allows the reader to fill out a modern approach to structural science and connects the organization of image data and imaging data analytics with the physics of actually obtaining a cellular image.
| Original language | English |
|---|---|
| Title of host publication | Pathobiology of Human Disease |
| Subtitle of host publication | A Dynamic Encyclopedia of Disease Mechanisms |
| Publisher | Elsevier Inc. |
| Pages | 3723-3759 |
| Number of pages | 37 |
| ISBN (Electronic) | 9780123864567 |
| ISBN (Print) | 9780123864574 |
| DOIs | |
| State | Published - Jan 1 2014 |
Keywords
- AmpLitude objects
- Anisotropic
- Birefringent
- Confocal laser scanning microscopy
- Contrast
- Deconvolution imaging
- Deconvolution microscopy
- Depth of field
- Dichroism
- Differential interference contrast microscope
- Epifluorescence microscope
- Finite bright-field microscope
- Fluorescence in situ hybridization
- Fluorescence resonance energy transfer
- Fluorescent analogs
- Fluorescent proteins
- Fluorochrome
- Fluorophore
- Infinity corrected bright-field microscope
- Isotropic
- Köhler illumination
- Laser capture microdissection
- Multiphoton microscopy
- Phase objects
- Photobleaching
- Polarizing microscope
- Quantitative histocytometry
- Quantum dots
- Quenching
- Resolution
- Spinning disk confocal microscopy
- Super-resolution/locaLization microscopy
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