An ordered noti fragment map of human chromosome band 11p15

M. J. Higgins; N. J. Smilinich; S. Sait; A. Koenig; J. Pongratz; M. Gessler; C. W. Richard; M. R. James; J. P. Sanford; B. W. Kim; J. Cattelane; N. J. Nowak; A. Winterpacht; B. U. Zabel; D. J. Munroe; E. Bric; D. E. Housman; C. Jones; Y. Nakamura; D. S. Gerhard; T. B. Shows

doi:10.1006/geno.1994.1479

An ordered noti fragment map of human chromosome band 11p15

M. J. Higgins
, N. J. Smilinich
, S. Sait
, A. Koenig
, J. Pongratz
, M. Gessler
, C. W. Richard
, M. R. James
, J. P. Sanford
, B. W. Kim
, J. Cattelane
, N. J. Nowak
, A. Winterpacht
, B. U. Zabel
, D. J. Munroe
, E. Bric
, D. E. Housman
, C. Jones
, Y. Nakamura
, D. S. Gerhard

T. B. Shows

Biochemistry

Roswell Park Cancer Institute
University of Marburg
University of Würzburg
University of Pittsburgh
Institut national de la santé et de la recherche médicale
Johannes Gutenberg University Mainz
Massachusetts Institute of Technology
University of Denver
Japanese Foundation for Cancer Research
Washington University St. Louis

Research output: Contribution to journal › Article › peer-review

26 Scopus citations

Abstract

An ordered NotI fragment map containing over 60 loci and encompassing approximately 17 Mb has been constructed for human chromosome band 11p15. Forty-two probes, including 11 NotI-linking cosmids, were subregionally mapped to 11p15 using a subset of the J1-deletion hybrids. These and 23 other probes defining loci previously mapped to 11p15 were hybridized to genomic DNA digested with NotI and 5 other infrequently cleaving restriction enzymes and separated by pulsed-field gel electrophoresis. Thirty-nine distinct NotI fragments were detected encompassing approximately 85% of the estimated length of 11p15. The predicted order of the gene loci used is cen-MYOD1-PTH- CALCA-ST5-RBTN1-HPX-HBB-RRM1-TH/INS/IGF2-H19-CTSD-MUC2-DRD4-HRAS-RNH-tel. This map will allow higher resolution mapping of new 11p15 markers, facilitate positional cloning of disease genes, and provide a framework for the physical mapping of 11p15 in clone contigs.

Original language	English
Pages (from-to)	211-222
Number of pages	12
Journal	Genomics
Volume	23
Issue number	1
DOIs	https://doi.org/10.1006/geno.1994.1479
State	Published - Sep 1994

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Higgins, M. J., Smilinich, N. J., Sait, S., Koenig, A., Pongratz, J., Gessler, M., Richard, C. W., James, M. R., Sanford, J. P., Kim, B. W., Cattelane, J., Nowak, N. J., Winterpacht, A., Zabel, B. U., Munroe, D. J., Bric, E., Housman, D. E., Jones, C., Nakamura, Y., ... Shows, T. B. (1994). An ordered noti fragment map of human chromosome band 11p15. Genomics, 23(1), 211-222. https://doi.org/10.1006/geno.1994.1479

@article{58dedaecef944d1eb6764ee829a2b68d,

title = "An ordered noti fragment map of human chromosome band 11p15",

abstract = "An ordered NotI fragment map containing over 60 loci and encompassing approximately 17 Mb has been constructed for human chromosome band 11p15. Forty-two probes, including 11 NotI-linking cosmids, were subregionally mapped to 11p15 using a subset of the J1-deletion hybrids. These and 23 other probes defining loci previously mapped to 11p15 were hybridized to genomic DNA digested with NotI and 5 other infrequently cleaving restriction enzymes and separated by pulsed-field gel electrophoresis. Thirty-nine distinct NotI fragments were detected encompassing approximately 85\% of the estimated length of 11p15. The predicted order of the gene loci used is cen-MYOD1-PTH- CALCA-ST5-RBTN1-HPX-HBB-RRM1-TH/INS/IGF2-H19-CTSD-MUC2-DRD4-HRAS-RNH-tel. This map will allow higher resolution mapping of new 11p15 markers, facilitate positional cloning of disease genes, and provide a framework for the physical mapping of 11p15 in clone contigs.",

author = "Higgins, \{M. J.\} and Smilinich, \{N. J.\} and S. Sait and A. Koenig and J. Pongratz and M. Gessler and Richard, \{C. W.\} and James, \{M. R.\} and Sanford, \{J. P.\} and Kim, \{B. W.\} and J. Cattelane and Nowak, \{N. J.\} and A. Winterpacht and Zabel, \{B. U.\} and Munroe, \{D. J.\} and E. Bric and Housman, \{D. E.\} and C. Jones and Y. Nakamura and Gerhard, \{D. S.\} and Shows, \{T. B.\}",

year = "1994",

month = sep,

doi = "10.1006/geno.1994.1479",

language = "English",

volume = "23",

pages = "211--222",

journal = "Genomics",

issn = "0888-7543",

publisher = "Academic Press Inc.",

number = "1",

}

Higgins, MJ, Smilinich, NJ, Sait, S, Koenig, A, Pongratz, J, Gessler, M, Richard, CW, James, MR, Sanford, JP, Kim, BW, Cattelane, J, Nowak, NJ, Winterpacht, A, Zabel, BU, Munroe, DJ, Bric, E, Housman, DE, Jones, C, Nakamura, Y, Gerhard, DS & Shows, TB 1994, 'An ordered noti fragment map of human chromosome band 11p15', Genomics, vol. 23, no. 1, pp. 211-222. https://doi.org/10.1006/geno.1994.1479

TY - JOUR

T1 - An ordered noti fragment map of human chromosome band 11p15

AU - Higgins, M. J.

AU - Smilinich, N. J.

AU - Sait, S.

AU - Koenig, A.

AU - Pongratz, J.

AU - Gessler, M.

AU - Richard, C. W.

AU - James, M. R.

AU - Sanford, J. P.

AU - Kim, B. W.

AU - Cattelane, J.

AU - Nowak, N. J.

AU - Winterpacht, A.

AU - Zabel, B. U.

AU - Munroe, D. J.

AU - Bric, E.

AU - Housman, D. E.

AU - Jones, C.

AU - Nakamura, Y.

AU - Gerhard, D. S.

AU - Shows, T. B.

PY - 1994/9

Y1 - 1994/9

N2 - An ordered NotI fragment map containing over 60 loci and encompassing approximately 17 Mb has been constructed for human chromosome band 11p15. Forty-two probes, including 11 NotI-linking cosmids, were subregionally mapped to 11p15 using a subset of the J1-deletion hybrids. These and 23 other probes defining loci previously mapped to 11p15 were hybridized to genomic DNA digested with NotI and 5 other infrequently cleaving restriction enzymes and separated by pulsed-field gel electrophoresis. Thirty-nine distinct NotI fragments were detected encompassing approximately 85% of the estimated length of 11p15. The predicted order of the gene loci used is cen-MYOD1-PTH- CALCA-ST5-RBTN1-HPX-HBB-RRM1-TH/INS/IGF2-H19-CTSD-MUC2-DRD4-HRAS-RNH-tel. This map will allow higher resolution mapping of new 11p15 markers, facilitate positional cloning of disease genes, and provide a framework for the physical mapping of 11p15 in clone contigs.

AB - An ordered NotI fragment map containing over 60 loci and encompassing approximately 17 Mb has been constructed for human chromosome band 11p15. Forty-two probes, including 11 NotI-linking cosmids, were subregionally mapped to 11p15 using a subset of the J1-deletion hybrids. These and 23 other probes defining loci previously mapped to 11p15 were hybridized to genomic DNA digested with NotI and 5 other infrequently cleaving restriction enzymes and separated by pulsed-field gel electrophoresis. Thirty-nine distinct NotI fragments were detected encompassing approximately 85% of the estimated length of 11p15. The predicted order of the gene loci used is cen-MYOD1-PTH- CALCA-ST5-RBTN1-HPX-HBB-RRM1-TH/INS/IGF2-H19-CTSD-MUC2-DRD4-HRAS-RNH-tel. This map will allow higher resolution mapping of new 11p15 markers, facilitate positional cloning of disease genes, and provide a framework for the physical mapping of 11p15 in clone contigs.

UR - https://www.scopus.com/pages/publications/0028145407

U2 - 10.1006/geno.1994.1479

DO - 10.1006/geno.1994.1479

M3 - Article

AN - SCOPUS:0028145407

SN - 0888-7543

VL - 23

SP - 211

EP - 222

JO - Genomics

JF - Genomics

IS - 1

ER -

An ordered noti fragment map of human chromosome band 11p15

Abstract

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