An integrated microfluidic biochemical detection system with magnetic bead-based sampling and analysis capabilities

Jin Woo Choi; Kwang W. Oh; Jennifer H. Thomas; William R. Heineman; H. Brian Halsall; Joseph H. Nevin; Arthur J. Helmicki; H. Thurman Henderson; Chong H. Ahn

doi:10.1109/MEMSYS.2001.906574

An integrated microfluidic biochemical detection system with magnetic bead-based sampling and analysis capabilities

Jin Woo Choi
, Kwang W. Oh
, Jennifer H. Thomas
, William R. Heineman
, H. Brian Halsall
, Joseph H. Nevin
, Arthur J. Helmicki
, H. Thurman Henderson
, Chong H. Ahn

Department of Electrical Engineering

University of Cincinnati

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

This paper presents the development and characterization of an integrated microfluidic biochemical detection system for fast and low volume immunoassays using magnetic beads, which are used as both immobilization surfaces and bio-molecule carriers. Magnetic bead-based immunoassay, as a typical example of biochemical detection and analysis, has been successfully performed on the integrated microfluidic biochemical analysis system that includes a surface-mounted biofilter and immunosensor on a glass microfluidic motherboard. Total time required for full immunoassay was less than 20 minutes including sample incubation time and sample volume wasted was less than 50 μl during five repeated assays. Fast and low volume biochemical analysis has been successfully achieved with the developed biofilter and immunosensor, which is integrated to microfluidic system.

Original language	English
Pages (from-to)	447-450
Number of pages	4
Journal	Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
DOIs	https://doi.org/10.1109/MEMSYS.2001.906574
State	Published - 2001

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Choi, J. W., Oh, K. W., Thomas, J. H., Heineman, W. R., Halsall, H. B., Nevin, J. H., Helmicki, A. J., Henderson, H. T., & Ahn, C. H. (2001). An integrated microfluidic biochemical detection system with magnetic bead-based sampling and analysis capabilities. Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS), 447-450. https://doi.org/10.1109/MEMSYS.2001.906574

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title = "An integrated microfluidic biochemical detection system with magnetic bead-based sampling and analysis capabilities",

abstract = "This paper presents the development and characterization of an integrated microfluidic biochemical detection system for fast and low volume immunoassays using magnetic beads, which are used as both immobilization surfaces and bio-molecule carriers. Magnetic bead-based immunoassay, as a typical example of biochemical detection and analysis, has been successfully performed on the integrated microfluidic biochemical analysis system that includes a surface-mounted biofilter and immunosensor on a glass microfluidic motherboard. Total time required for full immunoassay was less than 20 minutes including sample incubation time and sample volume wasted was less than 50 μl during five repeated assays. Fast and low volume biochemical analysis has been successfully achieved with the developed biofilter and immunosensor, which is integrated to microfluidic system.",

author = "Choi, \{Jin Woo\} and Oh, \{Kwang W.\} and Thomas, \{Jennifer H.\} and Heineman, \{William R.\} and Halsall, \{H. Brian\} and Nevin, \{Joseph H.\} and Helmicki, \{Arthur J.\} and Henderson, \{H. Thurman\} and Ahn, \{Chong H.\}",

year = "2001",

doi = "10.1109/MEMSYS.2001.906574",

language = "English",

pages = "447--450",

journal = "Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)",

issn = "1084-6999",

publisher = "Institute of Electrical and Electronics Engineers Inc.",

}

Choi, JW, Oh, KW, Thomas, JH, Heineman, WR, Halsall, HB, Nevin, JH, Helmicki, AJ, Henderson, HT & Ahn, CH 2001, 'An integrated microfluidic biochemical detection system with magnetic bead-based sampling and analysis capabilities', Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS), pp. 447-450. https://doi.org/10.1109/MEMSYS.2001.906574

TY - JOUR

T1 - An integrated microfluidic biochemical detection system with magnetic bead-based sampling and analysis capabilities

AU - Choi, Jin Woo

AU - Oh, Kwang W.

AU - Thomas, Jennifer H.

AU - Heineman, William R.

AU - Halsall, H. Brian

AU - Nevin, Joseph H.

AU - Helmicki, Arthur J.

AU - Henderson, H. Thurman

AU - Ahn, Chong H.

PY - 2001

Y1 - 2001

N2 - This paper presents the development and characterization of an integrated microfluidic biochemical detection system for fast and low volume immunoassays using magnetic beads, which are used as both immobilization surfaces and bio-molecule carriers. Magnetic bead-based immunoassay, as a typical example of biochemical detection and analysis, has been successfully performed on the integrated microfluidic biochemical analysis system that includes a surface-mounted biofilter and immunosensor on a glass microfluidic motherboard. Total time required for full immunoassay was less than 20 minutes including sample incubation time and sample volume wasted was less than 50 μl during five repeated assays. Fast and low volume biochemical analysis has been successfully achieved with the developed biofilter and immunosensor, which is integrated to microfluidic system.

AB - This paper presents the development and characterization of an integrated microfluidic biochemical detection system for fast and low volume immunoassays using magnetic beads, which are used as both immobilization surfaces and bio-molecule carriers. Magnetic bead-based immunoassay, as a typical example of biochemical detection and analysis, has been successfully performed on the integrated microfluidic biochemical analysis system that includes a surface-mounted biofilter and immunosensor on a glass microfluidic motherboard. Total time required for full immunoassay was less than 20 minutes including sample incubation time and sample volume wasted was less than 50 μl during five repeated assays. Fast and low volume biochemical analysis has been successfully achieved with the developed biofilter and immunosensor, which is integrated to microfluidic system.

UR - https://www.scopus.com/pages/publications/0035008299

U2 - 10.1109/MEMSYS.2001.906574

DO - 10.1109/MEMSYS.2001.906574

M3 - Article

AN - SCOPUS:0035008299

SN - 1084-6999

SP - 447

EP - 450

JO - Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)

JF - Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)

ER -

An integrated microfluidic biochemical detection system with magnetic bead-based sampling and analysis capabilities

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