TY - GEN
T1 - An integrated centrifugal degassed pdms-based microfluidic device for serial dilution
AU - Wang, Anyang
AU - Boroujeni, Samaneh Moghadasi
AU - Andreadis, Stelios T.
AU - Oh, Kwang W.
N1 - Publisher Copyright:
© 2020 CBMS-0001
PY - 2020
Y1 - 2020
N2 - In this paper, dilution series generations using a centrifugal force with a degassed PDMS made microfluidic device were proposed. The vacuum-driven force allows the device avoiding air bubbles as well as high tolerance in the surface condition, which was used for sample metering and sample transferring. In addition, centrifugal force was used for sample loading, into designate chambers to perform serial dilution as well as sample mixing. Quantitative Polymerase Chain Reaction (qPCR) was demonstrated as a proof of concept.
AB - In this paper, dilution series generations using a centrifugal force with a degassed PDMS made microfluidic device were proposed. The vacuum-driven force allows the device avoiding air bubbles as well as high tolerance in the surface condition, which was used for sample metering and sample transferring. In addition, centrifugal force was used for sample loading, into designate chambers to perform serial dilution as well as sample mixing. Quantitative Polymerase Chain Reaction (qPCR) was demonstrated as a proof of concept.
KW - Centrifugal microfluidic
KW - QPCR
KW - Serial dilution
KW - Vacuum-driven pumping
UR - https://www.scopus.com/pages/publications/85098246963
M3 - Conference contribution
AN - SCOPUS:85098246963
T3 - MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences
SP - 713
EP - 714
BT - MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PB - Chemical and Biological Microsystems Society
T2 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2020
Y2 - 4 October 2020 through 9 October 2020
ER -