Adsorption of human salivary mucins to hydroxyapatite

L. A. Tabak; M. J. Levine; N. K. Jain; Amy R. Bryan; R. E. Cohen; Laura D. Monte; S. Zawacki; G. H. Nancollas; Amalia Slomiany; B. L. Slomiany

doi:10.1016/0003-9969(85)90070-6

Adsorption of human salivary mucins to hydroxyapatite

L. A. Tabak
, M. J. Levine
, N. K. Jain
, Amy R. Bryan
, R. E. Cohen
, Laura D. Monte
, S. Zawacki
, G. H. Nancollas
, Amalia Slomiany
, B. L. Slomiany

SUNY Buffalo
New York Medical College

Research output: Contribution to journal › Article › peer-review

76 Scopus citations

Abstract

The interactions between low (MG2) and high (MG1) molecular-weight human submandibular-sublingual mucin and hydroxyapatite (OHAp) were compared using a quantitative assay. Data obtained appeared to empirically fit the Langmuir adsorption isotherm. Apparent affinity constants derived from this isotherm indicated that MG1 had a greater affinity for OHAp than did MG2. Inhibition studies revealed that salivary glycolipids inhibited the interaction of MG1 and OHAp without influencing MG2 adsorption. In contrast, MG2 adsorption to OHAp was markedly inhibited by cysteine-containing salivary phosphoprotein fractions. Collectively, the data indicate MG1 and MG2 differ in their interaction with OHAp.

Original language	English
Pages (from-to)	423-427
Number of pages	5
Journal	Archives of Oral Biology
Volume	30
Issue number	5
DOIs	https://doi.org/10.1016/0003-9969(85)90070-6
State	Published - 1985

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@article{75ae0117ce3b49d6b6238a4c26337e7c,

title = "Adsorption of human salivary mucins to hydroxyapatite",

abstract = "The interactions between low (MG2) and high (MG1) molecular-weight human submandibular-sublingual mucin and hydroxyapatite (OHAp) were compared using a quantitative assay. Data obtained appeared to empirically fit the Langmuir adsorption isotherm. Apparent affinity constants derived from this isotherm indicated that MG1 had a greater affinity for OHAp than did MG2. Inhibition studies revealed that salivary glycolipids inhibited the interaction of MG1 and OHAp without influencing MG2 adsorption. In contrast, MG2 adsorption to OHAp was markedly inhibited by cysteine-containing salivary phosphoprotein fractions. Collectively, the data indicate MG1 and MG2 differ in their interaction with OHAp.",

author = "Tabak, \{L. A.\} and Levine, \{M. J.\} and Jain, \{N. K.\} and Bryan, \{Amy R.\} and Cohen, \{R. E.\} and Monte, \{Laura D.\} and S. Zawacki and Nancollas, \{G. H.\} and Amalia Slomiany and Slomiany, \{B. L.\}",

year = "1985",

doi = "10.1016/0003-9969(85)90070-6",

language = "English",

volume = "30",

pages = "423--427",

journal = "Archives of Oral Biology",

issn = "0003-9969",

publisher = "Elsevier Ltd",

number = "5",

}

TY - JOUR

T1 - Adsorption of human salivary mucins to hydroxyapatite

AU - Tabak, L. A.

AU - Levine, M. J.

AU - Jain, N. K.

AU - Bryan, Amy R.

AU - Cohen, R. E.

AU - Monte, Laura D.

AU - Zawacki, S.

AU - Nancollas, G. H.

AU - Slomiany, Amalia

AU - Slomiany, B. L.

PY - 1985

Y1 - 1985

N2 - The interactions between low (MG2) and high (MG1) molecular-weight human submandibular-sublingual mucin and hydroxyapatite (OHAp) were compared using a quantitative assay. Data obtained appeared to empirically fit the Langmuir adsorption isotherm. Apparent affinity constants derived from this isotherm indicated that MG1 had a greater affinity for OHAp than did MG2. Inhibition studies revealed that salivary glycolipids inhibited the interaction of MG1 and OHAp without influencing MG2 adsorption. In contrast, MG2 adsorption to OHAp was markedly inhibited by cysteine-containing salivary phosphoprotein fractions. Collectively, the data indicate MG1 and MG2 differ in their interaction with OHAp.

AB - The interactions between low (MG2) and high (MG1) molecular-weight human submandibular-sublingual mucin and hydroxyapatite (OHAp) were compared using a quantitative assay. Data obtained appeared to empirically fit the Langmuir adsorption isotherm. Apparent affinity constants derived from this isotherm indicated that MG1 had a greater affinity for OHAp than did MG2. Inhibition studies revealed that salivary glycolipids inhibited the interaction of MG1 and OHAp without influencing MG2 adsorption. In contrast, MG2 adsorption to OHAp was markedly inhibited by cysteine-containing salivary phosphoprotein fractions. Collectively, the data indicate MG1 and MG2 differ in their interaction with OHAp.

UR - https://www.scopus.com/pages/publications/17544399630

U2 - 10.1016/0003-9969(85)90070-6

DO - 10.1016/0003-9969(85)90070-6

M3 - Article

C2 - 2992430

AN - SCOPUS:17544399630

SN - 0003-9969

VL - 30

SP - 423

EP - 427

JO - Archives of Oral Biology

JF - Archives of Oral Biology

IS - 5

ER -

Adsorption of human salivary mucins to hydroxyapatite

Abstract

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