ACTH-induced prostaglandin biosynthesis from 3H-arachidonic acid by adrenocortical cells

S. G. Laychock; R. P. Rubin

doi:10.1016/0090-6980(75)90135-5

ACTH-induced prostaglandin biosynthesis from ³H-arachidonic acid by adrenocortical cells

S. G. Laychock
, R. P. Rubin

Pharmacology and Toxicology

Virginia Commonwealth University

Research output: Contribution to journal › Article › peer-review

39 Scopus citations

Abstract

Prostaglandins biosynthesized from ³H-arachidonic acid by trypsin-dispersed cat adrenocortical cells were isolated by silicic acid and thin layer chromatography. PGE, PGF, and a third component with mobility properties indistinguishable from either PGA or PGB were identified both in cortical cell homogenates and incubation medium. Concentrations of ACTH (125-250 μU) which stimulate steroidogenesis enhanced the conversion of labeled precursor to all three of these prostaglandins. These findings provide further evidence for the proposal that prostaglandins function as a critical link in ACTH-induced steroidogenesis.

Original language	English
Pages (from-to)	529-540
Number of pages	12
Journal	Prostaglandins
Volume	10
Issue number	3
DOIs	https://doi.org/10.1016/0090-6980(75)90135-5
State	Published - Sep 1975

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title = "ACTH-induced prostaglandin biosynthesis from 3H-arachidonic acid by adrenocortical cells",

abstract = "Prostaglandins biosynthesized from 3H-arachidonic acid by trypsin-dispersed cat adrenocortical cells were isolated by silicic acid and thin layer chromatography. PGE, PGF, and a third component with mobility properties indistinguishable from either PGA or PGB were identified both in cortical cell homogenates and incubation medium. Concentrations of ACTH (125-250 μU) which stimulate steroidogenesis enhanced the conversion of labeled precursor to all three of these prostaglandins. These findings provide further evidence for the proposal that prostaglandins function as a critical link in ACTH-induced steroidogenesis.",

author = "Laychock, \{S. G.\} and Rubin, \{R. P.\}",

year = "1975",

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AU - Laychock, S. G.

AU - Rubin, R. P.

PY - 1975/9

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N2 - Prostaglandins biosynthesized from 3H-arachidonic acid by trypsin-dispersed cat adrenocortical cells were isolated by silicic acid and thin layer chromatography. PGE, PGF, and a third component with mobility properties indistinguishable from either PGA or PGB were identified both in cortical cell homogenates and incubation medium. Concentrations of ACTH (125-250 μU) which stimulate steroidogenesis enhanced the conversion of labeled precursor to all three of these prostaglandins. These findings provide further evidence for the proposal that prostaglandins function as a critical link in ACTH-induced steroidogenesis.

AB - Prostaglandins biosynthesized from 3H-arachidonic acid by trypsin-dispersed cat adrenocortical cells were isolated by silicic acid and thin layer chromatography. PGE, PGF, and a third component with mobility properties indistinguishable from either PGA or PGB were identified both in cortical cell homogenates and incubation medium. Concentrations of ACTH (125-250 μU) which stimulate steroidogenesis enhanced the conversion of labeled precursor to all three of these prostaglandins. These findings provide further evidence for the proposal that prostaglandins function as a critical link in ACTH-induced steroidogenesis.

UR - https://www.scopus.com/pages/publications/0016802389

U2 - 10.1016/0090-6980(75)90135-5

DO - 10.1016/0090-6980(75)90135-5

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AN - SCOPUS:0016802389

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SP - 529

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JO - Prostaglandins

JF - Prostaglandins

IS - 3

ER -

ACTH-induced prostaglandin biosynthesis from ³H-arachidonic acid by adrenocortical cells

Abstract

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