A simple method for fabrication of microarrays and microfluidic device using PDMS stamp

We propose a simple approach to fabricate a series of microwell arrays using the conventional PDMS (Polydimethylsiloxane) stamp approach to study the cell-to- cell adhesion of Human Bone Marrow Mesenchymal Stem Cells (BM-MSCs). This approach can be applied to fabricate a microfluidic channel with glass-PDMS-glass configuration for cytologic diagnosis. To replicate the microwell arrays and the microfluidic channel, an SU-8 master mold is created, and then a PDMS stamp replica is transferred to a glass substrate. For the pattern transfer from the PDMS stamp to the glass substrate, the surface of the PDMS stamp is treated by oxygen plasma and bonded on the glass substrate. By ripping the PDMS stamp by means of applying a mechanical force, the PDMS patterns are formed on the glass substrate. For the cell-to-cell interaction studies, 4 different types of PDMS arrays are created to vary cell-to-cell contact length. For the cytologic diagnosis, the microchannel is designed, that allows for the enrichment and temporary immobilization of the cells.