A simple and robust fabrication of microwell array by PDMS on a glass substrate for cell-to-cell adhesion

Hun Lee; Sindhu Row; Linfeng Xu; Stelios T. Andreadis; Kwang W. Oh

A simple and robust fabrication of microwell array by PDMS on a glass substrate for cell-to-cell adhesion

Hun Lee
, Sindhu Row
, Linfeng Xu
, Stelios T. Andreadis
, Kwang W. Oh

SUNY Buffalo

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

We propose a simple approach to fabricate a microwell array by using Polydimethylsiloxane (PDMS) to study cell-to-cell interaction of Human Bone Marrow Mesenchymal Stem Cells (BM-MSCs). The micro well array of PDMS is replicated from an SU-8 master mold, and then is transferred to a glass substrate by ripping the array that is bonded to the glass substrate by an oxygen plasma treatment. 4 different types of PDMS array (e.g., rectangle, bowtie, wide-rhombus, and rhombus) are formed to vary cell-to-cell contact length. The pattern transfer success rate and applied force are investigated. This method of generating the microwell array will enable a simple and robust construction of PDMS array for various cell-to-cell contact studies.

Original language	English
Title of host publication	Technical Proceedings of the 2014 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2014
Publisher	Nano Science and Technology Institute
Pages	145-148
Number of pages	4
ISBN (Print)	9781482258271
State	Published - 2014
Event	Nanotechnology 2014: MEMS, Fluidics, Bio Systems, Medical, Computational and Photonics - 2014 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2014 - Washington, DC, United States Duration: Jun 15 2014 → Jun 18 2014

Publication series

Name	Technical Proceedings of the 2014 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2014
Volume	2

Conference

Conference	Nanotechnology 2014: MEMS, Fluidics, Bio Systems, Medical, Computational and Photonics - 2014 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2014
Country/Territory	United States
City	Washington, DC
Period	06/15/14 → 06/18/14

Keywords

Cell-to-cell adhesion
Pattern transfer
PDMS microwell array

Cite this

Lee, H., Row, S., Xu, L., Andreadis, S. T., & Oh, K. W. (2014). A simple and robust fabrication of microwell array by PDMS on a glass substrate for cell-to-cell adhesion. In Technical Proceedings of the 2014 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2014 (pp. 145-148). (Technical Proceedings of the 2014 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2014; Vol. 2). Nano Science and Technology Institute.

Lee, Hun ; Row, Sindhu ; Xu, Linfeng et al. / A simple and robust fabrication of microwell array by PDMS on a glass substrate for cell-to-cell adhesion. Technical Proceedings of the 2014 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2014. Nano Science and Technology Institute, 2014. pp. 145-148 (Technical Proceedings of the 2014 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2014).

@inproceedings{f1403bb78ed84e07baf3deb9bca28197,

title = "A simple and robust fabrication of microwell array by PDMS on a glass substrate for cell-to-cell adhesion",

abstract = "We propose a simple approach to fabricate a microwell array by using Polydimethylsiloxane (PDMS) to study cell-to-cell interaction of Human Bone Marrow Mesenchymal Stem Cells (BM-MSCs). The micro well array of PDMS is replicated from an SU-8 master mold, and then is transferred to a glass substrate by ripping the array that is bonded to the glass substrate by an oxygen plasma treatment. 4 different types of PDMS array (e.g., rectangle, bowtie, wide-rhombus, and rhombus) are formed to vary cell-to-cell contact length. The pattern transfer success rate and applied force are investigated. This method of generating the microwell array will enable a simple and robust construction of PDMS array for various cell-to-cell contact studies.",

keywords = "Cell-to-cell adhesion, Pattern transfer, PDMS microwell array",

author = "Hun Lee and Sindhu Row and Linfeng Xu and Andreadis, \{Stelios T.\} and Oh, \{Kwang W.\}",

year = "2014",

language = "English",

isbn = "9781482258271",

series = "Technical Proceedings of the 2014 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2014",

publisher = "Nano Science and Technology Institute",

pages = "145--148",

booktitle = "Technical Proceedings of the 2014 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2014",

note = "Nanotechnology 2014: MEMS, Fluidics, Bio Systems, Medical, Computational and Photonics - 2014 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2014 ; Conference date: 15-06-2014 Through 18-06-2014",

}

Lee, H, Row, S, Xu, L, Andreadis, ST & Oh, KW 2014, A simple and robust fabrication of microwell array by PDMS on a glass substrate for cell-to-cell adhesion. in Technical Proceedings of the 2014 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2014. Technical Proceedings of the 2014 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2014, vol. 2, Nano Science and Technology Institute, pp. 145-148, Nanotechnology 2014: MEMS, Fluidics, Bio Systems, Medical, Computational and Photonics - 2014 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2014, Washington, DC, United States, 06/15/14.

A simple and robust fabrication of microwell array by PDMS on a glass substrate for cell-to-cell adhesion. / Lee, Hun; Row, Sindhu; Xu, Linfeng et al.
Technical Proceedings of the 2014 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2014. Nano Science and Technology Institute, 2014. p. 145-148 (Technical Proceedings of the 2014 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2014; Vol. 2).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - A simple and robust fabrication of microwell array by PDMS on a glass substrate for cell-to-cell adhesion

AU - Lee, Hun

AU - Row, Sindhu

AU - Xu, Linfeng

AU - Andreadis, Stelios T.

AU - Oh, Kwang W.

PY - 2014

Y1 - 2014

N2 - We propose a simple approach to fabricate a microwell array by using Polydimethylsiloxane (PDMS) to study cell-to-cell interaction of Human Bone Marrow Mesenchymal Stem Cells (BM-MSCs). The micro well array of PDMS is replicated from an SU-8 master mold, and then is transferred to a glass substrate by ripping the array that is bonded to the glass substrate by an oxygen plasma treatment. 4 different types of PDMS array (e.g., rectangle, bowtie, wide-rhombus, and rhombus) are formed to vary cell-to-cell contact length. The pattern transfer success rate and applied force are investigated. This method of generating the microwell array will enable a simple and robust construction of PDMS array for various cell-to-cell contact studies.

AB - We propose a simple approach to fabricate a microwell array by using Polydimethylsiloxane (PDMS) to study cell-to-cell interaction of Human Bone Marrow Mesenchymal Stem Cells (BM-MSCs). The micro well array of PDMS is replicated from an SU-8 master mold, and then is transferred to a glass substrate by ripping the array that is bonded to the glass substrate by an oxygen plasma treatment. 4 different types of PDMS array (e.g., rectangle, bowtie, wide-rhombus, and rhombus) are formed to vary cell-to-cell contact length. The pattern transfer success rate and applied force are investigated. This method of generating the microwell array will enable a simple and robust construction of PDMS array for various cell-to-cell contact studies.

KW - Cell-to-cell adhesion

KW - Pattern transfer

KW - PDMS microwell array

UR - https://www.scopus.com/pages/publications/84907386673

M3 - Conference contribution

AN - SCOPUS:84907386673

SN - 9781482258271

T3 - Technical Proceedings of the 2014 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2014

SP - 145

EP - 148

BT - Technical Proceedings of the 2014 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2014

PB - Nano Science and Technology Institute

T2 - Nanotechnology 2014: MEMS, Fluidics, Bio Systems, Medical, Computational and Photonics - 2014 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2014

Y2 - 15 June 2014 through 18 June 2014

ER -

Lee H, Row S, Xu L, Andreadis ST , Oh KW. A simple and robust fabrication of microwell array by PDMS on a glass substrate for cell-to-cell adhesion. In Technical Proceedings of the 2014 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2014. Nano Science and Technology Institute. 2014. p. 145-148. (Technical Proceedings of the 2014 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2014).

A simple and robust fabrication of microwell array by PDMS on a glass substrate for cell-to-cell adhesion

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Keywords

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