A real-time PCR assay for rapid detection and quantification of Exserohilum rostratum, a causative pathogen of fungal meningitis associated with injection of contaminated methylprednisolone

Yanan Zhao; Ruta Petraitiene; Thomas J. Walsh; David S. Perlin

doi:10.1128/JCM.03369-12

A real-time PCR assay for rapid detection and quantification of Exserohilum rostratum, a causative pathogen of fungal meningitis associated with injection of contaminated methylprednisolone

Yanan Zhao
, Ruta Petraitiene
, Thomas J. Walsh
, David S. Perlin

Pharmacy

New York Presbyterian Hospital
Public Health Research Institute, New York

Research output: Contribution to journal › Article › peer-review

30 Scopus citations

Abstract

A species-specific molecular beacon real-time PCR assay was developed for rapid diagnosis of Exserohilum rostratum infection. As low as 100 fg of E. rostratum DNA can be reliably detected in the presence of 50 ng of human DNA, with a dynamic linear quantification range from 20 ng to 200 fg.

Original language	English
Pages (from-to)	1034-1036
Number of pages	3
Journal	Journal of Clinical Microbiology
Volume	51
Issue number	3
DOIs	https://doi.org/10.1128/JCM.03369-12
State	Published - Mar 2013

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10.1128/JCM.03369-12

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title = "A real-time PCR assay for rapid detection and quantification of Exserohilum rostratum, a causative pathogen of fungal meningitis associated with injection of contaminated methylprednisolone",

abstract = "A species-specific molecular beacon real-time PCR assay was developed for rapid diagnosis of Exserohilum rostratum infection. As low as 100 fg of E. rostratum DNA can be reliably detected in the presence of 50 ng of human DNA, with a dynamic linear quantification range from 20 ng to 200 fg.",

author = "Yanan Zhao and Ruta Petraitiene and Walsh, \{Thomas J.\} and Perlin, \{David S.\}",

year = "2013",

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language = "English",

volume = "51",

pages = "1034--1036",

journal = "Journal of Clinical Microbiology",

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A real-time PCR assay for rapid detection and quantification of Exserohilum rostratum, a causative pathogen of fungal meningitis associated with injection of contaminated methylprednisolone. / Zhao, Yanan; Petraitiene, Ruta; Walsh, Thomas J. et al.
In: Journal of Clinical Microbiology, Vol. 51, No. 3, 03.2013, p. 1034-1036.

Research output: Contribution to journal › Article › peer-review

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AU - Perlin, David S.

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AB - A species-specific molecular beacon real-time PCR assay was developed for rapid diagnosis of Exserohilum rostratum infection. As low as 100 fg of E. rostratum DNA can be reliably detected in the presence of 50 ng of human DNA, with a dynamic linear quantification range from 20 ng to 200 fg.

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A real-time PCR assay for rapid detection and quantification of Exserohilum rostratum, a causative pathogen of fungal meningitis associated with injection of contaminated methylprednisolone

Abstract

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