A next-generation proteome array for Schistosoma mansoni

Rafael Ramiro de Assis; Fernanda Ludolf; Rie Nakajima; Al Jasinskas; Guilherme C. Oliveira; Philip L. Felgner; Soraya T. Gaze; Alex Loukas; Philip T. LoVerde; Jeffrey M. Bethony; Rodrigo Correa-Oliveira; Carlos E. Calzavara-Silva

doi:10.1016/j.ijpara.2016.04.001

A next-generation proteome array for Schistosoma mansoni

Rafael Ramiro de Assis
, Fernanda Ludolf
, Rie Nakajima
, Al Jasinskas
, Guilherme C. Oliveira
, Philip L. Felgner
, Soraya T. Gaze
, Alex Loukas
, Philip T. LoVerde
, Jeffrey M. Bethony
, Rodrigo Correa-Oliveira
, Carlos E. Calzavara-Silva

Microbiology and Immunology

Fundação Oswaldo Cruz
University of California at Irvine
Universidade Federal de Minas Gerais
Instituto Tecnológico Vale
James Cook University Queensland
George Washington University

Research output: Contribution to journal › Article › peer-review

24 Scopus citations

Abstract

A proteome microarray consisting of 992 Schistosoma mansoni proteins was produced and screened with sera to determine antibody signatures indicative of the clinical stages of schistosomiasis and the identification of subunit vaccine candidates. Herein, we describe the methods used to derive the gene list for this array (representing approximately 10% of the predicted S. mansoni proteome). We also probed a pilot version of the microarray with sera from individuals either acutely or chronically infected with S. mansoni from endemic areas in Brazil and sera from individuals resident outside the endemic area (USA) to determine if the array is functional and informative.

Original language	English
Pages (from-to)	411-415
Number of pages	5
Journal	International Journal for Parasitology
Volume	46
Issue number	7
DOIs	https://doi.org/10.1016/j.ijpara.2016.04.001
State	Published - Jun 1 2016

Keywords

Antibody signature
Protein microarray
Proteome
Schistosoma mansoni

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10.1016/j.ijpara.2016.04.001

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title = "A next-generation proteome array for Schistosoma mansoni",

abstract = "A proteome microarray consisting of 992 Schistosoma mansoni proteins was produced and screened with sera to determine antibody signatures indicative of the clinical stages of schistosomiasis and the identification of subunit vaccine candidates. Herein, we describe the methods used to derive the gene list for this array (representing approximately 10\% of the predicted S. mansoni proteome). We also probed a pilot version of the microarray with sera from individuals either acutely or chronically infected with S. mansoni from endemic areas in Brazil and sera from individuals resident outside the endemic area (USA) to determine if the array is functional and informative.",

keywords = "Antibody signature, Protein microarray, Proteome, Schistosoma mansoni",

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doi = "10.1016/j.ijpara.2016.04.001",

language = "English",

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AU - de Assis, Rafael Ramiro

AU - Ludolf, Fernanda

AU - Nakajima, Rie

AU - Jasinskas, Al

AU - Oliveira, Guilherme C.

AU - Felgner, Philip L.

AU - Gaze, Soraya T.

AU - Loukas, Alex

AU - LoVerde, Philip T.

AU - Bethony, Jeffrey M.

AU - Correa-Oliveira, Rodrigo

AU - Calzavara-Silva, Carlos E.

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AB - A proteome microarray consisting of 992 Schistosoma mansoni proteins was produced and screened with sera to determine antibody signatures indicative of the clinical stages of schistosomiasis and the identification of subunit vaccine candidates. Herein, we describe the methods used to derive the gene list for this array (representing approximately 10% of the predicted S. mansoni proteome). We also probed a pilot version of the microarray with sera from individuals either acutely or chronically infected with S. mansoni from endemic areas in Brazil and sera from individuals resident outside the endemic area (USA) to determine if the array is functional and informative.

KW - Antibody signature

KW - Protein microarray

KW - Proteome

KW - Schistosoma mansoni

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ER -

A next-generation proteome array for Schistosoma mansoni

Abstract

Keywords

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