A new method for assaying adhesion of cancer cells to the greater omentum and its application for evaluating anti-adhesion activities of chemically synthesized oligosaccharides

Akihiko Okamura; Shin Yazawa; Toyo Nishimura; Susumu Tanaka; Izumi Takai; Shigeharu Kudo; Takayuki Asao; Hiroyuki Kuwano; Khushi L. Matta; Suguru Akamatsu; Naohisa Kochibe

doi:10.1023/A:1026526829010

A new method for assaying adhesion of cancer cells to the greater omentum and its application for evaluating anti-adhesion activities of chemically synthesized oligosaccharides

Akihiko Okamura
, Shin Yazawa
, Toyo Nishimura
, Susumu Tanaka
, Izumi Takai
, Shigeharu Kudo
, Takayuki Asao
, Hiroyuki Kuwano
, Khushi L. Matta
, Suguru Akamatsu
, Naohisa Kochibe

Department of Chemical and Biological Engineering

Gunma University
Otsuka Pharmaceutical Co Ltd.
Roswell Park Cancer Institute

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

A new ex vivo method for assaying adhesion of cancer cells to the greater omentum has been developed using mouse greater omentum and [³H]labelled human gastric and mouse colorectal cancer cells. Since the adhesion rates were found to increase up to 18 h and labelled cells seemed to be stable during the period, the present method could be useful for investigating adhesion of cancer cells to the greater omentum, which must occur at the first step of the peritoneal dissemination. The adhesion of cancer cells to the greater omentum was inhibited by a series of chemically synthesized oligosaccharides and Galβ1,3[3OMeGalβ1,4GlcNAcβ1,6]αBn was found to be the best inhibitor. The anti-tumor effect of this novel tetrasaccharide in vivo was shown in preliminary experiments using Balb/c mice and colon26 cells.

Original language	English
Pages (from-to)	37-43
Number of pages	7
Journal	Clinical and Experimental Metastasis
Volume	18
Issue number	1
DOIs	https://doi.org/10.1023/A:1026526829010
State	Published - 2000

Keywords

Anti-adhesion therapy
Cell adhesion
Chemically synthesized oligosaccharide
Fucosyltransferase
Greater omentum
Peritoneal dissemination

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10.1023/A:1026526829010

Cite this

Okamura, A., Yazawa, S., Nishimura, T., Tanaka, S., Takai, I., Kudo, S., Asao, T., Kuwano, H., Matta, K. L., Akamatsu, S., & Kochibe, N. (2000). A new method for assaying adhesion of cancer cells to the greater omentum and its application for evaluating anti-adhesion activities of chemically synthesized oligosaccharides. Clinical and Experimental Metastasis, 18(1), 37-43. https://doi.org/10.1023/A:1026526829010

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title = "A new method for assaying adhesion of cancer cells to the greater omentum and its application for evaluating anti-adhesion activities of chemically synthesized oligosaccharides",

abstract = "A new ex vivo method for assaying adhesion of cancer cells to the greater omentum has been developed using mouse greater omentum and [3H]labelled human gastric and mouse colorectal cancer cells. Since the adhesion rates were found to increase up to 18 h and labelled cells seemed to be stable during the period, the present method could be useful for investigating adhesion of cancer cells to the greater omentum, which must occur at the first step of the peritoneal dissemination. The adhesion of cancer cells to the greater omentum was inhibited by a series of chemically synthesized oligosaccharides and Galβ1,3[3OMeGalβ1,4GlcNAcβ1,6]αBn was found to be the best inhibitor. The anti-tumor effect of this novel tetrasaccharide in vivo was shown in preliminary experiments using Balb/c mice and colon26 cells.",

keywords = "Anti-adhesion therapy, Cell adhesion, Chemically synthesized oligosaccharide, Fucosyltransferase, Greater omentum, Peritoneal dissemination",

author = "Akihiko Okamura and Shin Yazawa and Toyo Nishimura and Susumu Tanaka and Izumi Takai and Shigeharu Kudo and Takayuki Asao and Hiroyuki Kuwano and Matta, \{Khushi L.\} and Suguru Akamatsu and Naohisa Kochibe",

year = "2000",

doi = "10.1023/A:1026526829010",

language = "English",

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pages = "37--43",

journal = "Clinical and Experimental Metastasis",

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Okamura, A, Yazawa, S, Nishimura, T, Tanaka, S, Takai, I, Kudo, S, Asao, T, Kuwano, H, Matta, KL, Akamatsu, S & Kochibe, N 2000, 'A new method for assaying adhesion of cancer cells to the greater omentum and its application for evaluating anti-adhesion activities of chemically synthesized oligosaccharides', Clinical and Experimental Metastasis, vol. 18, no. 1, pp. 37-43. https://doi.org/10.1023/A:1026526829010

A new method for assaying adhesion of cancer cells to the greater omentum and its application for evaluating anti-adhesion activities of chemically synthesized oligosaccharides. / Okamura, Akihiko; Yazawa, Shin; Nishimura, Toyo et al.
In: Clinical and Experimental Metastasis, Vol. 18, No. 1, 2000, p. 37-43.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - A new method for assaying adhesion of cancer cells to the greater omentum and its application for evaluating anti-adhesion activities of chemically synthesized oligosaccharides

AU - Okamura, Akihiko

AU - Yazawa, Shin

AU - Nishimura, Toyo

AU - Tanaka, Susumu

AU - Takai, Izumi

AU - Kudo, Shigeharu

AU - Asao, Takayuki

AU - Kuwano, Hiroyuki

AU - Matta, Khushi L.

AU - Akamatsu, Suguru

AU - Kochibe, Naohisa

PY - 2000

Y1 - 2000

N2 - A new ex vivo method for assaying adhesion of cancer cells to the greater omentum has been developed using mouse greater omentum and [3H]labelled human gastric and mouse colorectal cancer cells. Since the adhesion rates were found to increase up to 18 h and labelled cells seemed to be stable during the period, the present method could be useful for investigating adhesion of cancer cells to the greater omentum, which must occur at the first step of the peritoneal dissemination. The adhesion of cancer cells to the greater omentum was inhibited by a series of chemically synthesized oligosaccharides and Galβ1,3[3OMeGalβ1,4GlcNAcβ1,6]αBn was found to be the best inhibitor. The anti-tumor effect of this novel tetrasaccharide in vivo was shown in preliminary experiments using Balb/c mice and colon26 cells.

AB - A new ex vivo method for assaying adhesion of cancer cells to the greater omentum has been developed using mouse greater omentum and [3H]labelled human gastric and mouse colorectal cancer cells. Since the adhesion rates were found to increase up to 18 h and labelled cells seemed to be stable during the period, the present method could be useful for investigating adhesion of cancer cells to the greater omentum, which must occur at the first step of the peritoneal dissemination. The adhesion of cancer cells to the greater omentum was inhibited by a series of chemically synthesized oligosaccharides and Galβ1,3[3OMeGalβ1,4GlcNAcβ1,6]αBn was found to be the best inhibitor. The anti-tumor effect of this novel tetrasaccharide in vivo was shown in preliminary experiments using Balb/c mice and colon26 cells.

KW - Anti-adhesion therapy

KW - Cell adhesion

KW - Chemically synthesized oligosaccharide

KW - Fucosyltransferase

KW - Greater omentum

KW - Peritoneal dissemination

UR - https://www.scopus.com/pages/publications/17744388104

U2 - 10.1023/A:1026526829010

DO - 10.1023/A:1026526829010

M3 - Article

C2 - 11206836

AN - SCOPUS:17744388104

SN - 0262-0898

VL - 18

SP - 37

EP - 43

JO - Clinical and Experimental Metastasis

JF - Clinical and Experimental Metastasis

IS - 1

ER -

A new method for assaying adhesion of cancer cells to the greater omentum and its application for evaluating anti-adhesion activities of chemically synthesized oligosaccharides

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Keywords

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