A microfluidic assay for measuring electrical conductivity of gap junction channels

Cedric Bathany; Frederick Sachs; Susan Z. Hua

doi:10.1557/opl.2012.43

A microfluidic assay for measuring electrical conductivity of gap junction channels

Cedric Bathany
, Frederick Sachs
, Susan Z. Hua

Department of Mechanical and Aerospace Engineering

SUNY Buffalo

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

We have developed a microfluidic sensor to measure the electrical coupling through gap junctions across a 2D sheet of cultured Normal Rat Kidney cells. The chip is based on a tri-stream laminar flow with conductive solutions on either end and a nonconductive solution in the middle stream. When an electrical voltage is applied, the current can only pass through the cell sheet, thereby enabling us to measure the electrical coupling of gap junctions between cells. Using this sensor we have measured the effect of 2-aminoethoxydiphenyl borate (2-APB) on Cx43 channels. We show that 2-APB reversibly inhibits electrical coupling of Cx43 in NRK cells. Moreover, we screened other potential candidates to block Cx43, 1-Heptanol and GsMTx-4, and examined their effects on Cx43 gap junctions.

Original language	English
Title of host publication	MEMS, BioMEMS and Bioelectronics - Materials and Devices
Pages	67-73
Number of pages	7
DOIs	https://doi.org/10.1557/opl.2012.43
State	Published - 2012
Event	2011 MRS Fall Meeting - Boston, MA, United States Duration: Nov 28 2011 → Dec 2 2012

Publication series

Name	Materials Research Society Symposium Proceedings
Volume	1415
ISSN (Print)	0272-9172

Conference

Conference	2011 MRS Fall Meeting
Country/Territory	United States
City	Boston, MA
Period	11/28/11 → 12/2/12

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10.1557/opl.2012.43

Cite this

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title = "A microfluidic assay for measuring electrical conductivity of gap junction channels",

abstract = "We have developed a microfluidic sensor to measure the electrical coupling through gap junctions across a 2D sheet of cultured Normal Rat Kidney cells. The chip is based on a tri-stream laminar flow with conductive solutions on either end and a nonconductive solution in the middle stream. When an electrical voltage is applied, the current can only pass through the cell sheet, thereby enabling us to measure the electrical coupling of gap junctions between cells. Using this sensor we have measured the effect of 2-aminoethoxydiphenyl borate (2-APB) on Cx43 channels. We show that 2-APB reversibly inhibits electrical coupling of Cx43 in NRK cells. Moreover, we screened other potential candidates to block Cx43, 1-Heptanol and GsMTx-4, and examined their effects on Cx43 gap junctions.",

author = "Cedric Bathany and Frederick Sachs and Hua, \{Susan Z.\}",

year = "2012",

doi = "10.1557/opl.2012.43",

language = "English",

isbn = "9781605113920",

series = "Materials Research Society Symposium Proceedings",

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A microfluidic assay for measuring electrical conductivity of gap junction channels. / Bathany, Cedric; Sachs, Frederick; Hua, Susan Z.
MEMS, BioMEMS and Bioelectronics - Materials and Devices. 2012. p. 67-73 (Materials Research Society Symposium Proceedings; Vol. 1415).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

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AU - Bathany, Cedric

AU - Sachs, Frederick

AU - Hua, Susan Z.

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N2 - We have developed a microfluidic sensor to measure the electrical coupling through gap junctions across a 2D sheet of cultured Normal Rat Kidney cells. The chip is based on a tri-stream laminar flow with conductive solutions on either end and a nonconductive solution in the middle stream. When an electrical voltage is applied, the current can only pass through the cell sheet, thereby enabling us to measure the electrical coupling of gap junctions between cells. Using this sensor we have measured the effect of 2-aminoethoxydiphenyl borate (2-APB) on Cx43 channels. We show that 2-APB reversibly inhibits electrical coupling of Cx43 in NRK cells. Moreover, we screened other potential candidates to block Cx43, 1-Heptanol and GsMTx-4, and examined their effects on Cx43 gap junctions.

AB - We have developed a microfluidic sensor to measure the electrical coupling through gap junctions across a 2D sheet of cultured Normal Rat Kidney cells. The chip is based on a tri-stream laminar flow with conductive solutions on either end and a nonconductive solution in the middle stream. When an electrical voltage is applied, the current can only pass through the cell sheet, thereby enabling us to measure the electrical coupling of gap junctions between cells. Using this sensor we have measured the effect of 2-aminoethoxydiphenyl borate (2-APB) on Cx43 channels. We show that 2-APB reversibly inhibits electrical coupling of Cx43 in NRK cells. Moreover, we screened other potential candidates to block Cx43, 1-Heptanol and GsMTx-4, and examined their effects on Cx43 gap junctions.

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SN - 9781605113920

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A microfluidic assay for measuring electrical conductivity of gap junction channels

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