@inbook{ad3b05d35bb84e51b8af57d660cc93b7,
title = "A hybrid biological–biomaterial vector for antigen delivery",
abstract = "A hybrid biological–biomaterial vector composed of a biocompatible polymeric biomaterial coating and an Escherichia coli core was designed and developed for antigen delivery. It provides a unique and efficient mechanism to transport antigens (protein or genetic) via different mechanisms of vector design that include antigen cellular localization (cytoplasm, periplasm, cellular surface) and nonnative functionalities that assist in antigen delivery. Based on a variety of E. coli strain development and polymer chemistry tools, the hybrid vector can be constructed into a number of formats for the purpose of optimized uptake and processing by antigen presenting cells, serving as the basis for a potent subsequent immune response. This chapter serves to outline a protocol for assembling a hybrid biological–biomaterial vector for use as a vaccine delivery system.",
keywords = "Biomaterial, DNA vaccine, Escherichia coli, Immunogenicity, Vaccine delivery",
author = "Ruiquan Qi and Andrew Hill and Pfeifer, \{Blaine A.\}",
note = "Publisher Copyright: {\textcopyright} Springer Science+Business Media, LLC, part of Springer Nature 2021.",
year = "2021",
doi = "10.1007/978-1-0716-0795-4\_25",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "461--475",
booktitle = "Methods in Molecular Biology",
}