A deliberate approach to screening for initial crystallization conditions of biological macromolecules

Joseph R. Luft; Robert J. Collins; Nancy A. Fehrman; Angela M. Lauricella; Christina K. Veatch; George T. DeTitta

doi:10.1016/S1047-8477(03)00048-0

A deliberate approach to screening for initial crystallization conditions of biological macromolecules

Joseph R. Luft
, Robert J. Collins
, Nancy A. Fehrman
, Angela M. Lauricella
, Christina K. Veatch
, George T. DeTitta

Department of Structural Biology

Hauptman-Woodward Medical Research Institute, Inc.
SUNY Buffalo

Research output: Contribution to journal › Article › peer-review

276 Scopus citations

Abstract

A method to rationally predict crystallization conditions for a previously uncrystallized macromolecule has not yet been developed. One way around this problem is to determine initial crystallization conditions by casting a wide net, surveying a large number of chemical and physical conditions to locate crystallization leads. A facility that executes the rapid survey of crystallization lead conditions is described in detail. Results and guidelines for the initial screening of crystallization conditions, applicable to both manual and robotic setups, are discussed.

Original language	English
Pages (from-to)	170-179
Number of pages	10
Journal	Journal of Structural Biology
Volume	142
Issue number	1
DOIs	https://doi.org/10.1016/S1047-8477(03)00048-0
State	Published - Apr 1 2003

Keywords

Crystallization
High throughput
Macromolecule
Microbatch

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10.1016/S1047-8477(03)00048-0

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abstract = "A method to rationally predict crystallization conditions for a previously uncrystallized macromolecule has not yet been developed. One way around this problem is to determine initial crystallization conditions by casting a wide net, surveying a large number of chemical and physical conditions to locate crystallization leads. A facility that executes the rapid survey of crystallization lead conditions is described in detail. Results and guidelines for the initial screening of crystallization conditions, applicable to both manual and robotic setups, are discussed.",

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AU - Veatch, Christina K.

AU - DeTitta, George T.

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KW - Crystallization

KW - High throughput

KW - Macromolecule

KW - Microbatch

UR - https://www.scopus.com/pages/publications/0037394492

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A deliberate approach to screening for initial crystallization conditions of biological macromolecules

Abstract

Keywords

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