A cholinergic antagonist blocks cold stress-induced alterations in rat adrenal tyrosine hydroxylase mRNA

Michal Stachowiak; Edward M. Stricker; Michael J. Zigmond; Barry B. Kaplan

doi:10.1016/0169-328X(88)90066-6

A cholinergic antagonist blocks cold stress-induced alterations in rat adrenal tyrosine hydroxylase mRNA

Michal Stachowiak
, Edward M. Stricker
, Michael J. Zigmond
, Barry B. Kaplan

Department of Pathology and Anatomical Sciences

University of Pittsburgh

Research output: Contribution to journal › Article › peer-review

43 Scopus citations

Abstract

The role of nicotinic cholinergic transmission in cold stress-induced alterations in rat adrenomedullary tyrosine hydroxylase (TH) mRNA was investigated by RNA dot-blot hybridization, using a cloned TH cDNA probe. Chlorisondamine, a ganglionic blocking agent, greatly attenuated the induction of TH mRNA levels caused by cold exposure, whereas carbachol and nicotine, cholinergic agonists, increased TH mRNA in control animals. These results suggest that cholinergic nicotinic receptors play a key role in the trans-synaptic induction of adrenal TH gene expression.

Original language	English
Pages (from-to)	193-195
Number of pages	3
Journal	Molecular Brain Research
Volume	3
Issue number	2
DOIs	https://doi.org/10.1016/0169-328X(88)90066-6
State	Published - Apr 1988

Keywords

Adrenal gland
Chlorisondamine
Cholinomimetics
Cold stress
Tyrosine hydroxylase mRNA

Access to Document

10.1016/0169-328X(88)90066-6

Cite this

@article{1b7df6a9aff4491fb851e6b4c73dcbe7,

title = "A cholinergic antagonist blocks cold stress-induced alterations in rat adrenal tyrosine hydroxylase mRNA",

abstract = "The role of nicotinic cholinergic transmission in cold stress-induced alterations in rat adrenomedullary tyrosine hydroxylase (TH) mRNA was investigated by RNA dot-blot hybridization, using a cloned TH cDNA probe. Chlorisondamine, a ganglionic blocking agent, greatly attenuated the induction of TH mRNA levels caused by cold exposure, whereas carbachol and nicotine, cholinergic agonists, increased TH mRNA in control animals. These results suggest that cholinergic nicotinic receptors play a key role in the trans-synaptic induction of adrenal TH gene expression.",

keywords = "Adrenal gland, Chlorisondamine, Cholinomimetics, Cold stress, Tyrosine hydroxylase mRNA",

author = "Michal Stachowiak and Stricker, \{Edward M.\} and Zigmond, \{Michael J.\} and Kaplan, \{Barry B.\}",

year = "1988",

month = apr,

doi = "10.1016/0169-328X(88)90066-6",

language = "English",

volume = "3",

pages = "193--195",

journal = "Molecular Brain Research",

issn = "0169-328X",

publisher = "Elsevier B.V.",

number = "2",

}

TY - JOUR

T1 - A cholinergic antagonist blocks cold stress-induced alterations in rat adrenal tyrosine hydroxylase mRNA

AU - Stachowiak, Michal

AU - Stricker, Edward M.

AU - Zigmond, Michael J.

AU - Kaplan, Barry B.

PY - 1988/4

Y1 - 1988/4

N2 - The role of nicotinic cholinergic transmission in cold stress-induced alterations in rat adrenomedullary tyrosine hydroxylase (TH) mRNA was investigated by RNA dot-blot hybridization, using a cloned TH cDNA probe. Chlorisondamine, a ganglionic blocking agent, greatly attenuated the induction of TH mRNA levels caused by cold exposure, whereas carbachol and nicotine, cholinergic agonists, increased TH mRNA in control animals. These results suggest that cholinergic nicotinic receptors play a key role in the trans-synaptic induction of adrenal TH gene expression.

AB - The role of nicotinic cholinergic transmission in cold stress-induced alterations in rat adrenomedullary tyrosine hydroxylase (TH) mRNA was investigated by RNA dot-blot hybridization, using a cloned TH cDNA probe. Chlorisondamine, a ganglionic blocking agent, greatly attenuated the induction of TH mRNA levels caused by cold exposure, whereas carbachol and nicotine, cholinergic agonists, increased TH mRNA in control animals. These results suggest that cholinergic nicotinic receptors play a key role in the trans-synaptic induction of adrenal TH gene expression.

KW - Adrenal gland

KW - Chlorisondamine

KW - Cholinomimetics

KW - Cold stress

KW - Tyrosine hydroxylase mRNA

UR - https://www.scopus.com/pages/publications/0023916639

U2 - 10.1016/0169-328X(88)90066-6

DO - 10.1016/0169-328X(88)90066-6

M3 - Article

C2 - 2898270

AN - SCOPUS:0023916639

SN - 0169-328X

VL - 3

SP - 193

EP - 195

JO - Molecular Brain Research

JF - Molecular Brain Research

IS - 2

ER -

A cholinergic antagonist blocks cold stress-induced alterations in rat adrenal tyrosine hydroxylase mRNA

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this