A chemically labeled cytotoxic agent: Two-photon fluorophore for optical tracking of cellular pathway in chemotherapy

Xiaopeng Wang; Linda J. Krebs; Mohammed Al-Nuri; Haridas E. Pudavar; Saswati Ghosal; Charles Liebow; Attila A. Nagy; Andrew V. Schally; Paras N. Prasad

doi:10.1073/pnas.96.20.11081

A chemically labeled cytotoxic agent: Two-photon fluorophore for optical tracking of cellular pathway in chemotherapy

Xiaopeng Wang
, Linda J. Krebs
, Mohammed Al-Nuri
, Haridas E. Pudavar
, Saswati Ghosal
, Charles Liebow
, Attila A. Nagy
, Andrew V. Schally
, Paras N. Prasad

Chemistry

SUNY Buffalo
Women and Children's Hospital of Buffalo
An-Najah National University
Laser Photonics Technology, Inc.
Department of Veterans Affairs
Tulane University

Research output: Contribution to journal › Article › peer-review

105 Scopus citations

Abstract

Chemotherapy is commonly used in the treatment of cancers. However, the mechanism of action of many of these agents is not well understood. We present the synthesis of a two-photon fluorophore (C625) and its biological application when chemically linked to a chemotherapeutic agent (AN-152). By using two-photon laser-scanning microscopy, the drug:fluorophore conjugate can be observed directly as it interacts with receptor-positive cell lines. The results of this project visually show the receptor-mediated entry of AN- 152 into the cell cytoplasm and subsequently into the nucleus. These observations will allow for better understanding of the drug's therapeutic mechanism, which is a subject of ongoing research aimed at improving present methods for cancer therapy.

Original language	English
Pages (from-to)	11081-11084
Number of pages	4
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	96
Issue number	20
DOIs	https://doi.org/10.1073/pnas.96.20.11081
State	Published - Sep 28 1999

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10.1073/pnas.96.20.11081

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Wang, X., Krebs, L. J., Al-Nuri, M., Pudavar, H. E., Ghosal, S., Liebow, C., Nagy, A. A., Schally, A. V., & Prasad, P. N. (1999). A chemically labeled cytotoxic agent: Two-photon fluorophore for optical tracking of cellular pathway in chemotherapy. Proceedings of the National Academy of Sciences of the United States of America, 96(20), 11081-11084. https://doi.org/10.1073/pnas.96.20.11081

@article{2692c76829404859a12946435315d157,

title = "A chemically labeled cytotoxic agent: Two-photon fluorophore for optical tracking of cellular pathway in chemotherapy",

abstract = "Chemotherapy is commonly used in the treatment of cancers. However, the mechanism of action of many of these agents is not well understood. We present the synthesis of a two-photon fluorophore (C625) and its biological application when chemically linked to a chemotherapeutic agent (AN-152). By using two-photon laser-scanning microscopy, the drug:fluorophore conjugate can be observed directly as it interacts with receptor-positive cell lines. The results of this project visually show the receptor-mediated entry of AN- 152 into the cell cytoplasm and subsequently into the nucleus. These observations will allow for better understanding of the drug's therapeutic mechanism, which is a subject of ongoing research aimed at improving present methods for cancer therapy.",

author = "Xiaopeng Wang and Krebs, \{Linda J.\} and Mohammed Al-Nuri and Pudavar, \{Haridas E.\} and Saswati Ghosal and Charles Liebow and Nagy, \{Attila A.\} and Schally, \{Andrew V.\} and Prasad, \{Paras N.\}",

year = "1999",

month = sep,

day = "28",

doi = "10.1073/pnas.96.20.11081",

language = "English",

volume = "96",

pages = "11081--11084",

journal = "Proceedings of the National Academy of Sciences of the United States of America",

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Wang, X, Krebs, LJ, Al-Nuri, M, Pudavar, HE, Ghosal, S, Liebow, C, Nagy, AA, Schally, AV & Prasad, PN 1999, 'A chemically labeled cytotoxic agent: Two-photon fluorophore for optical tracking of cellular pathway in chemotherapy', Proceedings of the National Academy of Sciences of the United States of America, vol. 96, no. 20, pp. 11081-11084. https://doi.org/10.1073/pnas.96.20.11081

A chemically labeled cytotoxic agent: Two-photon fluorophore for optical tracking of cellular pathway in chemotherapy. / Wang, Xiaopeng; Krebs, Linda J.; Al-Nuri, Mohammed et al.
In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, No. 20, 28.09.1999, p. 11081-11084.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - A chemically labeled cytotoxic agent

T2 - Two-photon fluorophore for optical tracking of cellular pathway in chemotherapy

AU - Wang, Xiaopeng

AU - Krebs, Linda J.

AU - Al-Nuri, Mohammed

AU - Pudavar, Haridas E.

AU - Ghosal, Saswati

AU - Liebow, Charles

AU - Nagy, Attila A.

AU - Schally, Andrew V.

AU - Prasad, Paras N.

PY - 1999/9/28

Y1 - 1999/9/28

N2 - Chemotherapy is commonly used in the treatment of cancers. However, the mechanism of action of many of these agents is not well understood. We present the synthesis of a two-photon fluorophore (C625) and its biological application when chemically linked to a chemotherapeutic agent (AN-152). By using two-photon laser-scanning microscopy, the drug:fluorophore conjugate can be observed directly as it interacts with receptor-positive cell lines. The results of this project visually show the receptor-mediated entry of AN- 152 into the cell cytoplasm and subsequently into the nucleus. These observations will allow for better understanding of the drug's therapeutic mechanism, which is a subject of ongoing research aimed at improving present methods for cancer therapy.

AB - Chemotherapy is commonly used in the treatment of cancers. However, the mechanism of action of many of these agents is not well understood. We present the synthesis of a two-photon fluorophore (C625) and its biological application when chemically linked to a chemotherapeutic agent (AN-152). By using two-photon laser-scanning microscopy, the drug:fluorophore conjugate can be observed directly as it interacts with receptor-positive cell lines. The results of this project visually show the receptor-mediated entry of AN- 152 into the cell cytoplasm and subsequently into the nucleus. These observations will allow for better understanding of the drug's therapeutic mechanism, which is a subject of ongoing research aimed at improving present methods for cancer therapy.

UR - https://www.scopus.com/pages/publications/0033613144

U2 - 10.1073/pnas.96.20.11081

DO - 10.1073/pnas.96.20.11081

M3 - Article

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AN - SCOPUS:0033613144

SN - 0027-8424

VL - 96

SP - 11081

EP - 11084

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

IS - 20

ER -

A chemically labeled cytotoxic agent: Two-photon fluorophore for optical tracking of cellular pathway in chemotherapy

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