α-L-fucosidase from aspergillus niger: Demonstration of a novel α-L-(1 → 6)-fucosidase acting on glycopeptides

Shin Yazawa; Ragupathy Madiyalakan; Ramanik P. Chawda; Khushi L. Matta

doi:10.1016/0006-291X(86)90477-8

α-L-fucosidase from aspergillus niger: Demonstration of a novel α-L-(1 → 6)-fucosidase acting on glycopeptides

Shin Yazawa
, Ragupathy Madiyalakan
, Ramanik P. Chawda
, Khushi L. Matta

Department of Chemical and Biological Engineering

Roswell Park Cancer Institute

Research output: Contribution to journal › Article › peer-review

24 Scopus citations

Abstract

An α-L-fucosidase which hydrolyzes fucose from α-(1 → 6)-linkage to N-acetylglucosamine was found in Aspergillus niger. The enzyme was purified by affinity chromatography with bovine IgG glycopeptide-Sepharose 4B. The enzyme preparation released fucose from bovine IgG glycopeptide and fucosylated asialoagalactofetuin, but failed to cleave 1 → 2, 1 → 3 or 1 → 4 linkages of α-L-fucosides.

Original language	English
Pages (from-to)	563-569
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	136
Issue number	2
DOIs	https://doi.org/10.1016/0006-291X(86)90477-8
State	Published - Apr 29 1986

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title = "α-L-fucosidase from aspergillus niger: Demonstration of a novel α-L-(1 → 6)-fucosidase acting on glycopeptides",

abstract = "An α-L-fucosidase which hydrolyzes fucose from α-(1 → 6)-linkage to N-acetylglucosamine was found in Aspergillus niger. The enzyme was purified by affinity chromatography with bovine IgG glycopeptide-Sepharose 4B. The enzyme preparation released fucose from bovine IgG glycopeptide and fucosylated asialoagalactofetuin, but failed to cleave 1 → 2, 1 → 3 or 1 → 4 linkages of α-L-fucosides.",

author = "Shin Yazawa and Ragupathy Madiyalakan and Chawda, \{Ramanik P.\} and Matta, \{Khushi L.\}",

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AU - Yazawa, Shin

AU - Madiyalakan, Ragupathy

AU - Chawda, Ramanik P.

AU - Matta, Khushi L.

PY - 1986/4/29

Y1 - 1986/4/29

N2 - An α-L-fucosidase which hydrolyzes fucose from α-(1 → 6)-linkage to N-acetylglucosamine was found in Aspergillus niger. The enzyme was purified by affinity chromatography with bovine IgG glycopeptide-Sepharose 4B. The enzyme preparation released fucose from bovine IgG glycopeptide and fucosylated asialoagalactofetuin, but failed to cleave 1 → 2, 1 → 3 or 1 → 4 linkages of α-L-fucosides.

AB - An α-L-fucosidase which hydrolyzes fucose from α-(1 → 6)-linkage to N-acetylglucosamine was found in Aspergillus niger. The enzyme was purified by affinity chromatography with bovine IgG glycopeptide-Sepharose 4B. The enzyme preparation released fucose from bovine IgG glycopeptide and fucosylated asialoagalactofetuin, but failed to cleave 1 → 2, 1 → 3 or 1 → 4 linkages of α-L-fucosides.

UR - https://www.scopus.com/pages/publications/0022470344

U2 - 10.1016/0006-291X(86)90477-8

DO - 10.1016/0006-291X(86)90477-8

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ER -

α-L-fucosidase from aspergillus niger: Demonstration of a novel α-L-(1 → 6)-fucosidase acting on glycopeptides

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