Assessment of Neurotoxicity of Mixtures of PFAS and other Neuroactive Organic Pollutants through Integrated in Silico, in Vitro Cellular and in Vivo Models

This study will integratein vitroandin silicohigh throughput testing (HTT) within vivotests using zebrafish model to evaluate the neurotoxicity of mixtures of per- and polyfluoroalkyl substances (PFAS), and their mixtures with other organic contaminants. The role of cellular transporters in the active transport of PFAS will be evaluated to study the bioactivity of PFAS and their mixtures. These approaches will assess the role of PFAS in the etiology of neurodevelopmental disorders, particularly in the development of autism spectrum disorder (ASD).